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Part:BBa_K277085

Designed by: James DiCarlo   Group: iGEM09_Johns_Hopkins-BAG   (2009-10-21)


3L.3_23.C3.06

3L.3_23.C3.06 is 762 bases long and is cloned into the pGem-T vector.

3L.3_23.C3.06 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. The whole synthetic chromosome may be viewed at http://macbeth.clark.jhu.edu/cgi-bin/gbrowse 3L.3_23.C3.06 is a constituent of 3L.3_23.C3 (along with 3L.3_23.C3.01, 3L.3_23.C3.02, 3L.3_23.C3.03, 3L.3_23.C3.04, 3L.3_23.C3.05, 3L.3_23.C3.07, 3L.3_23.C3.08, 3L.3_23.C3.09, 3L.3_23.C3.10, 3L.3_23.C3.11, 3L.3_23.C3.12, 3L.3_23.C3.13, and 3L.3_23.C3.14.)

This part contains at least part of the following features (positions offset from first base of sequence):

kind and name offset notes

gene YCL027W (515..+2053) Membrane protein localized to the shmoo tip%2C required for cell fusion%3B expression regulated by mating pheromone%3B proposed to coordinate signaling%2C fusion%2C and polarization events required for fusion%3B potential Cdc28p substrate

mutation_affecting_coding_sequence YCL027W_re_remove_BlpI (698..706) removal of BlpI

loxP_site loxPsym_YCL028W (49..82)

gene YCL028W (-1172..45) %5BPIN(+)%5D prion%2C an infectious protein conformation that is generally an ordered protein aggregate

Sequence (corresponds to coordinates 61968..62729 in synthetic chromosome yeast_chr3_3_23)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 249
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 249
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 249
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 249
  • 1000
    COMPATIBLE WITH RFC[1000]


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